Related relative mRNA expression was calculated by the 2 2?Cq method and normalized to -actin [18]. was confirmed in MLE-12 cells Cell Death Detection Kit (Roche, USA) according to the manufacturers instructions. Briefly, sections (4?m) were deparaffinized in xylene, rehydrated in decreasing concentrations of ethanol, rehydrated and heated for antigen … Continue reading Related relative mRNA expression was calculated by the 2 2?Cq method and normalized to -actin [18]